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mock bacterial community controls  (Zymo Research)


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    Zymo Research mock bacterial community controls
    Mock Bacterial Community Controls, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 743 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mock+bacterial+community+controls/ZymoBIOMICS+Microbial+Community+DNA+Standard/pmc10750252-61-0-10
    Average 96 stars, based on 743 article reviews
    mock bacterial community controls - by Bioz Stars, 2026-10
    96/100 stars

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    other:

    Article Title: Association of Chlamydia trachomatis burden with the vaginal microbiota, bacterial vaginosis, and metronidazole treatment
    Article Snippet: Mock bacterial community controls (ZymoBIOMICS Microbial Community DNA Standard II, Zymo Research, Irvine, California) and environmental controls spiked with Thermus thermophilus HB8 genomic DNA (Takara Bio, San Jose, California) were sequenced in parallel to assess for background signals.



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    Clean reads of milk samples sequencing (V1V2 and V3V4) after applying different DNA extraction protocols (P1–P6, for description see Table ). All samples ( n = 4) were extracted in duplicate (P4 and P5) or in triplicate (P1, P2, P3, and P6). MOCK standard and NTC had clean reads of 21,812 and 545 in the V1V2 run and 33,142 and 3 in the V3V4 run, respectively (boxes not shown). Boxes include 25–75 percentile; whiskers include 5–95 percentile. Outliers not shown. + indicates the mean value; the line indicates the median value.

    Journal: MicrobiologyOpen

    Article Title: Bovine milk microbiota: Evaluation of different DNA extraction protocols for challenging samples

    doi: 10.1002/mbo3.1275

    Figure Lengend Snippet: Clean reads of milk samples sequencing (V1V2 and V3V4) after applying different DNA extraction protocols (P1–P6, for description see Table ). All samples ( n = 4) were extracted in duplicate (P4 and P5) or in triplicate (P1, P2, P3, and P6). MOCK standard and NTC had clean reads of 21,812 and 545 in the V1V2 run and 33,142 and 3 in the V3V4 run, respectively (boxes not shown). Boxes include 25–75 percentile; whiskers include 5–95 percentile. Outliers not shown. + indicates the mean value; the line indicates the median value.

    Article Snippet: A no‐template control (NTC) and a MOCK community DNA standard (Zymo Research Europe GmbH) with predetermined bacterial species were included ( Bacillus subtilis , Cryptococcus neoformans , Escherichia coli , Enterococcus faecalis , Listeria monocytogenes , Lactobacillus fermentum , Pseudomonas aeruginosa , Salmonella enterica , Staphylococcus aureus , Saccharomyces cerevisiae ).

    Techniques: Sequencing, DNA Extraction

    Clean reads of resequenced milk samples* with high/low cfu/SCC (V1V2 and V3V4) after applying different DNA extraction protocols (P3: QIAamp DNA Mini kit; P4, P6: modified DNeasy Blood & Tissue kit). All samples were extracted in two (P4) or three replicates (P3, P6) and sequenced in triplicates (1, 2, 3). *Prototype samples include unsuspicious milk (SCC−cfu−), and milk suspect of unspecific (SCC+cfu−; “high,” SCC+cfu+), or “latent” infection (SCC−cfu+). MOCK standard and NTC had clean reads of 29,222 and 0 in the V1V2 run and 23,300 and 224 in the V3V4 run, respectively (boxes not shown). cfu, Colony‐forming unit; NTC, no‐template control; rRNA, ribosomal RNA; SCC, somatic cell count.

    Journal: MicrobiologyOpen

    Article Title: Bovine milk microbiota: Evaluation of different DNA extraction protocols for challenging samples

    doi: 10.1002/mbo3.1275

    Figure Lengend Snippet: Clean reads of resequenced milk samples* with high/low cfu/SCC (V1V2 and V3V4) after applying different DNA extraction protocols (P3: QIAamp DNA Mini kit; P4, P6: modified DNeasy Blood & Tissue kit). All samples were extracted in two (P4) or three replicates (P3, P6) and sequenced in triplicates (1, 2, 3). *Prototype samples include unsuspicious milk (SCC−cfu−), and milk suspect of unspecific (SCC+cfu−; “high,” SCC+cfu+), or “latent” infection (SCC−cfu+). MOCK standard and NTC had clean reads of 29,222 and 0 in the V1V2 run and 23,300 and 224 in the V3V4 run, respectively (boxes not shown). cfu, Colony‐forming unit; NTC, no‐template control; rRNA, ribosomal RNA; SCC, somatic cell count.

    Article Snippet: A no‐template control (NTC) and a MOCK community DNA standard (Zymo Research Europe GmbH) with predetermined bacterial species were included ( Bacillus subtilis , Cryptococcus neoformans , Escherichia coli , Enterococcus faecalis , Listeria monocytogenes , Lactobacillus fermentum , Pseudomonas aeruginosa , Salmonella enterica , Staphylococcus aureus , Saccharomyces cerevisiae ).

    Techniques: DNA Extraction, Modification, Infection, Cell Counting